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hace2 receptor  (Addgene inc)


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    Addgene inc hace2 receptor
    Hace2 Receptor, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plenti+hace2+hygr/pLENTI_hACE2_HygR+(Plasmid+%23155296)/10__1016_slash_j__virol__2025__110659-55-25-30
    Average 94 stars, based on 24 article reviews
    hace2 receptor - by Bioz Stars, 2026-08
    94/100 stars

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    Addgene inc ace2
    SARS-CoV-2 receptors expression in SH-SY5Y. Cells were treated with CUR, EXT, Me08 or Me23 for 24 h and the gene expression was analyzed by RT-qPCR and protein expression was analyzed by Western-blotting. Gene expression of ( A ) <t>ACE2</t> ( B ) Furin, ( C ) TMPRSS2, ( D ) TMPRSS11D. Gene expression was normalized by the endogenous expression of RPL35. ( E ) Protein expression according to western blot analyses. ( F ) Histograms reporting the mean ± SD of ( G ) ACE2, ( H ) TMPRSS2, ( I ) TMPRSS11D levels after normalization with the average intensity of the bands from four independent experiments. Protein expression was normalized by the endogenous expression of GAPDH. CTL: cells without treatment, CUR: curcumin, EXT: extract. *p < 0.05. (n = 4–13). The entire blots are presented in Supplementary Figure S1.
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    SARS-CoV-2 receptors expression in SH-SY5Y. Cells were treated with CUR, EXT, Me08 or Me23 for 24 h and the gene expression was analyzed by RT-qPCR and protein expression was analyzed by Western-blotting. Gene expression of ( A ) <t>ACE2</t> ( B ) Furin, ( C ) TMPRSS2, ( D ) TMPRSS11D. Gene expression was normalized by the endogenous expression of RPL35. ( E ) Protein expression according to western blot analyses. ( F ) Histograms reporting the mean ± SD of ( G ) ACE2, ( H ) TMPRSS2, ( I ) TMPRSS11D levels after normalization with the average intensity of the bands from four independent experiments. Protein expression was normalized by the endogenous expression of GAPDH. CTL: cells without treatment, CUR: curcumin, EXT: extract. *p < 0.05. (n = 4–13). The entire blots are presented in Supplementary Figure S1.
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    SARS-CoV-2 receptors expression in SH-SY5Y. Cells were treated with CUR, EXT, Me08 or Me23 for 24 h and the gene expression was analyzed by RT-qPCR and protein expression was analyzed by Western-blotting. Gene expression of ( A ) ACE2 ( B ) Furin, ( C ) TMPRSS2, ( D ) TMPRSS11D. Gene expression was normalized by the endogenous expression of RPL35. ( E ) Protein expression according to western blot analyses. ( F ) Histograms reporting the mean ± SD of ( G ) ACE2, ( H ) TMPRSS2, ( I ) TMPRSS11D levels after normalization with the average intensity of the bands from four independent experiments. Protein expression was normalized by the endogenous expression of GAPDH. CTL: cells without treatment, CUR: curcumin, EXT: extract. *p < 0.05. (n = 4–13). The entire blots are presented in Supplementary Figure S1.

    Journal: Scientific Reports

    Article Title: Antiviral, anti-inflammatory and antioxidant effects of curcumin and curcuminoids in SH-SY5Y cells infected by SARS-CoV-2

    doi: 10.1038/s41598-024-61662-7

    Figure Lengend Snippet: SARS-CoV-2 receptors expression in SH-SY5Y. Cells were treated with CUR, EXT, Me08 or Me23 for 24 h and the gene expression was analyzed by RT-qPCR and protein expression was analyzed by Western-blotting. Gene expression of ( A ) ACE2 ( B ) Furin, ( C ) TMPRSS2, ( D ) TMPRSS11D. Gene expression was normalized by the endogenous expression of RPL35. ( E ) Protein expression according to western blot analyses. ( F ) Histograms reporting the mean ± SD of ( G ) ACE2, ( H ) TMPRSS2, ( I ) TMPRSS11D levels after normalization with the average intensity of the bands from four independent experiments. Protein expression was normalized by the endogenous expression of GAPDH. CTL: cells without treatment, CUR: curcumin, EXT: extract. *p < 0.05. (n = 4–13). The entire blots are presented in Supplementary Figure S1.

    Article Snippet: SH-SY5Y cells were transduced with a lentiviral vector to overexpress ACE2 (pLENTI-hACE2-HygR, Addgene #161758).

    Techniques: Expressing, Gene Expression, Quantitative RT-PCR, Western Blot

    ACE2 overexpression increases viral load in SH-SY5Y and potentialize the curcuminoids effect. ( A ) SH-SY5Y were transduced with a lentivector carrying the ACE2 gene and the cell line SH-ACE2 was created. The overexpression of ACE2 was evaluated by RT-qPCR. ( B ) SH-SY5Y and SH-ACE2 were treated with CUR, EXT, Me08 or Me23 for 2 h and after they were infected with SARS-CoV-2 (MOI 0.2) for 24 h. ( C ) The viral load was quantified by RTqPCR. CTL: Cells infected with SARS-CoV-2 and without treatment, CUR: curcumin, EXT: extract. **p < 0.01, ****p < 0.0001. (n = 3–6). The schematic diagram illustrating the infection process was based on a previous publication .

    Journal: Scientific Reports

    Article Title: Antiviral, anti-inflammatory and antioxidant effects of curcumin and curcuminoids in SH-SY5Y cells infected by SARS-CoV-2

    doi: 10.1038/s41598-024-61662-7

    Figure Lengend Snippet: ACE2 overexpression increases viral load in SH-SY5Y and potentialize the curcuminoids effect. ( A ) SH-SY5Y were transduced with a lentivector carrying the ACE2 gene and the cell line SH-ACE2 was created. The overexpression of ACE2 was evaluated by RT-qPCR. ( B ) SH-SY5Y and SH-ACE2 were treated with CUR, EXT, Me08 or Me23 for 2 h and after they were infected with SARS-CoV-2 (MOI 0.2) for 24 h. ( C ) The viral load was quantified by RTqPCR. CTL: Cells infected with SARS-CoV-2 and without treatment, CUR: curcumin, EXT: extract. **p < 0.01, ****p < 0.0001. (n = 3–6). The schematic diagram illustrating the infection process was based on a previous publication .

    Article Snippet: SH-SY5Y cells were transduced with a lentiviral vector to overexpress ACE2 (pLENTI-hACE2-HygR, Addgene #161758).

    Techniques: Over Expression, Transduction, Quantitative RT-PCR, Infection