Journal: Scientific Reports
Article Title: Antiviral, anti-inflammatory and antioxidant effects of curcumin and curcuminoids in SH-SY5Y cells infected by SARS-CoV-2
doi: 10.1038/s41598-024-61662-7
Figure Lengend Snippet: SARS-CoV-2 receptors expression in SH-SY5Y. Cells were treated with CUR, EXT, Me08 or Me23 for 24 h and the gene expression was analyzed by RT-qPCR and protein expression was analyzed by Western-blotting. Gene expression of ( A ) ACE2 ( B ) Furin, ( C ) TMPRSS2, ( D ) TMPRSS11D. Gene expression was normalized by the endogenous expression of RPL35. ( E ) Protein expression according to western blot analyses. ( F ) Histograms reporting the mean ± SD of ( G ) ACE2, ( H ) TMPRSS2, ( I ) TMPRSS11D levels after normalization with the average intensity of the bands from four independent experiments. Protein expression was normalized by the endogenous expression of GAPDH. CTL: cells without treatment, CUR: curcumin, EXT: extract. *p < 0.05. (n = 4–13). The entire blots are presented in Supplementary Figure S1.
Article Snippet: SH-SY5Y cells were transduced with a lentiviral vector to overexpress ACE2 (pLENTI-hACE2-HygR, Addgene #161758).
Techniques: Expressing, Gene Expression, Quantitative RT-PCR, Western Blot